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A Triple Primary Cell Culture Model of the Human Blood-Brain Barrier for Studying Ischemic Stroke In Vitro

3.8K Views

11:06 min

October 6th, 2022

DOI :

10.3791/64469-v

October 6th, 2022


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Triple Primary Cell Culture

Rozdziały w tym wideo

0:05

Introduction

0:50

Triple Cell Culture BBB Model Setting

3:35

Induction of the Oxygen-Glucose Deprivation

5:19

TEER Measurements

6:49

Assessment of the BBB Paracellular Permeability

7:40

Detection of Hypoxia in Live Cells

8:41

Results: A Method to Set up an In Vitro Reliable Triple Endothelial Cell-Pericyte-Astrocyte Culture BBB Model

10:20

Conclusion

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