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Three-dimensional Characterization of Interorganelle Contact Sites in Hepatocytes using Serial Section Electron Microscopy

5.5K Views

09:12 min

June 9th, 2022

DOI :

10.3791/63496-v

June 9th, 2022


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Three dimensional Characterization

Rozdziały w tym wideo

0:05

Introduction

1:08

Trimming and Serial Sectioning of Embedded Samples

4:12

Grid Staining

5:26

Imaging Acquisition by TEM

6:52

Segmentation and 3D Reconstruction

7:31

Results: Segmentation and 3D Reconstruction Reviewing the Morphology of Endoplasmic Reticulum and the Associated ER-Mitochondria Contacts

8:34

Conclusion

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