Visualizing Shifts on Neuron-Glia Circuit with the Calcium Imaging Technique
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4.4K Views
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11:41 min
•
April 8th, 2022
DOI :
April 8th, 2022
•1Laboratory of Neurochemistry, Institute of Biophysics Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, 2Laboratory of Neuroenergetics and Inborn Errors of Metabolism, Institute of Medical Biochemistry Leopoldo de Meis, Universidade Federal do Rio de Janeiro, 3Laboratory of Neurochemistry and Cell Biology, Institute of Life Sciences, Universidade Federal da Bahia
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Rozdziały w tym wideo
0:00
Introduction
0:59
Neurons or Glia?
1:30
Retina Dissection and Cell Culture Preparation
4:48
Loading Cells with FURA-2 AM
6:21
Calcium Imaging of Cells
9:10
Data Processing
9:38
Representative Results
10:50
Conclusion
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