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Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging

3.9K Views

06:44 min

April 28th, 2021

DOI :

10.3791/62553-v

April 28th, 2021


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DNA Double strand Break

Rozdziały w tym wideo

0:05

Introduction

0:37

Cell Culture, Treatments, and Coverslip Preparation

1:18

Pre-Extraction and Fixation

2:13

Permeabilization and Immunostaining

3:53

Image Analysis

4:16

Results: Visualization of DNA Double-Strand End Resection During S to G2 Phase of the Cell Cycle Using an Immunofluorescence-Based Method

6:05

Conclusion

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