Confocal Laser Scanning Microscopy of Calcium Dynamics in Acute Mouse Pancreatic Tissue Slices

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3.8K Views

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10:49 min

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April 13th, 2021

DOI :

10.3791/62293-v

April 13th, 2021

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Andraž Stožer¹, Jurij Dolenšek¹^,², Lidija Križančić Bombek¹, Viljem Pohorec¹, Marjan Slak Rupnik¹^,³, Maša Skelin Klemen¹

¹Institute of Physiology, Faculty of Medicine, University of Maribor, ²Faculty of Natural Sciences and Mathematics, University of Maribor, ³Center for Physiology and Pharmacology, Medical University of Vienna

Podsumowanie

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Confocal Laser Scanning Microscopy

Calcium Dynamics

Mouse Pancreatic Tissue

Live Cell Imaging

Intercellular Waves

Functional Connectivity

Tissue Architecture

Disease Progression

Pancreas related Diseases

Agarose Injection

Extracellular Solution

Euthanized Adult Mouse

Rozdziały w tym wideo

0:05

Introduction

1:04

Injection of Pancreas with Agarose

2:57

Tissue Slice Preparation

5:13

Calcium Dye Preparation and Loading

6:22

Calcium Imaging

8:17

Results: Calcium Dynamics in Acute Mouse Pancreatic Tissue Slices

10:03

Conclusion

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