Complementation of Splicing Activity by a Galectin-3 - U1 snRNP Complex on Beads
Please note that all translations are automatically generated. Click here for the English version.
2.1K Views
•
08:48 min
•
December 9th, 2020
DOI :
December 9th, 2020
•Rozdziały w tym wideo
0:04
Introduction
0:53
Preparation of NE Depleted of U1 snRNP (U1ΔNE)
2:10
Immunoprecipitation of 10S Fractions of Glycerol Gradients by Anti-Gal3
3:52
Assembly of Splicing Reaction and Analysis of Products
6:09
Results: RNA and Polypeptide Compositions of the Anti-Gal3 Precipitate of the 10S Region of the Glycerol Gradient and its Ability to Reconstitute Splicing in U1ΔNE
8:08
Conclusion
Powiązane Filmy
11:19
Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts
14.8K Views
10:06
Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
8.9K Views
11:34
Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
6.6K Views
08:12
Single-step Purification of Macromolecular Complexes Using RNA Attached to Biotin and a Photo-cleavable Linker
7.2K Views
00:11
Extremely Rapid and Specific Metabolic Labelling of RNA In Vivo with 4-Thiouracil (Ers4tU)
10.7K Views
10:25
Using the E1A Minigene Tool to Study mRNA Splicing Changes
4.7K Views
07:31
ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast
2.4K Views
05:44
Utilization of Grafix for the Detection of Transient Interactors of Saccharomyces cerevisiae Spliceosome Subcomplexes
3.5K Views
08:53
A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
2.6K Views
07:35
Analysis of Spliceosomal snRNA Localization in Human Hela Cells Using Microinjection
6.0K Views
Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone