Label-Free Imaging of Lipid Storage Dynamics in Caenorhabditis elegans using Stimulated Raman Scattering Microscopy

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4.1K Views

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10:59 min

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May 28th, 2021

DOI :

10.3791/61870-v

May 28th, 2021

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Ayse Sena Mutlu¹, Tao Chen¹, Dinghuan Deng¹, Meng C. Wang¹^,²^,³

¹Huffington Center on Aging, Baylor College of Medicine, ²Department of Molecular and Human Genetics, Baylor College of Medicine, ³Howard Hughes Medical Institute, Baylor College of Medicine

Podsumowanie

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Label Free Imaging

Lipid Storage Dynamics

Caenorhabditis Elegans

Stimulated Raman Scattering Microscopy

SRS Microscopy

Chemical Imaging Technology

Lipid Detection

Fluorescence Techniques

Lipid Biology

Laser Beam Alignment

Picosecond Light Source

Rozdziały w tym wideo

0:05

Introduction

0:51

Stimulated Raman Scattering (SRS) Microscopy Instrument Setup

3:53

Imaging Condition Optimization

5:21

C. elegans Sample Preparation

6:16

Image Acquisition and Analysis

7:26

Image Analysis

8:37

Results: Representative Effects of Insulin Signaling on Lipid Storage in C. elegans

10:03

Conclusion

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