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Purification of Tubulin with Controlled Posttranslational Modifications and Isotypes from Limited Sources by Polymerization-Depolymerization Cycles

5.0K Views

07:54 min

November 5th, 2020

DOI :

10.3791/61826-v

November 5th, 2020


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Tubulin Purification

Rozdziały w tym wideo

0:05

Introduction

0:34

Amplification and Lysis of Cells in Suspension Cultures

2:08

Amplification and Lysis of Adherent Cells and Mouse Brain Tissue Lysis

3:46

Sample Preparation for Analysis

4:10

Clarification, Polymerization, and Depolymerization of the Lysate

6:27

Evaluation, Quantification, and Characterization of Purified Tubulin

7:19

Conclusion

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