Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples
Please note that all translations are automatically generated. Click here for the English version.
5.0K Views
•
14:51 min
•
November 13th, 2021
DOI :
November 13th, 2021
•Rozdziały w tym wideo
0:00
Introduction
0:42
Tissue Lysate Preparation
1:51
Quantification and Quality Check of Tissue Lysates
2:33
Enzymatic Digestion of Proteins
3:57
Desalting of Digested Peptides
5:16
Quantification of Desalted Peptides
5:58
Labeling of Digested Peptides using iTRAQ Reagents
8:14
Liquid Chromatography Set-up
9:25
Mass Spectrometry Set-up
12:30
Representative Results
13:45
Conclusions
Powiązane Filmy
08:48
Quantitation of γH2AX Foci in Tissue Samples
14.3K Views
06:09
Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry
22.8K Views
09:20
Proteomic Sample Preparation from Formalin Fixed and Paraffin Embedded Tissue
27.5K Views
09:38
Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer
14.1K Views
14:23
Bottom-up and Shotgun Proteomics to Identify a Comprehensive Cochlear Proteome
18.6K Views
12:11
Capture Compound Mass Spectrometry - A Powerful Tool to Identify Novel c-di-GMP Effector Proteins
11.6K Views
09:49
Non-enzymatic, Serum-free Tissue Culture of Pre-invasive Breast Lesions for Spontaneous Generation of Mammospheres
12.5K Views
09:04
Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification
16.9K Views
07:28
Enrichment of Extracellular Matrix Proteins from Tissues and Digestion into Peptides for Mass Spectrometry Analysis
26.9K Views
10:40
Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine
10.4K Views
Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone