Enhanced Viability for Ex vivo 3D Hydrogel Cultures of Patient-Derived Xenografts in a Perfused Microfluidic Platform

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6.0K Views

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12:12 min

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December 5th, 2020

DOI :

10.3791/60872-v

December 5th, 2020

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Lindsey K. Sablatura¹, Kristin M. Bircsak², Peter Shepherd³, Karla Queiroz⁴, Mary C. Farach-Carson¹^,⁵^,⁶, Pamela E. Constantinou¹^,⁷, Anthony Saleh², Nora Navone³, Daniel A. Harrington¹^,⁵^,⁶

¹Department of BioSciences, Rice University, ²Mimetas US Inc, ³Department of Genitourinary Medical Oncology Research, Division of Cancer Medicine, The University of Texas MD Anderson Cancer Center, ⁴Mimetas B.V., ⁵Department of Bioengineering, Rice University, ⁶Department of Diagnostic and Biomedical Sciences, The University of Texas Health Science Center, ⁷Sheikh Ahmed Center for Pancreatic Cancer Research, The University of Texas MD Anderson Cancer Center

Podsumowanie

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3D Hydrogel Cultures

Patient derived Xenografts

Perfused Microfluidic Platform

PDX Processing Medium

Dissociation Enzyme Solution

Tumor Tissue Processing

Microfluidic Plates

Cell Density Estimation

Hemocytometer

Rozdziały w tym wideo

0:04

Introduction

1:09

PDX (Patient-Derived Xenografts) Dissociation and Initial Purification of Stromal Component

3:17

Density Gradient Centrifugation-Based Separation of PDX-Derived Clusters from Single Cells

6:03

Hydrogel Preparation and Microfluidic Plate Seeding

10:12

Results: Cell Staining and Viability Assessment

11:16

Conclusion

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