JoVE Journal

Bioengineering

Masz pełny dostęp do tej treści za pośrednictwem

Nanyang Technological University

Characterizing Single-Molecule Conformational Changes Under Shear Flow with Fluorescence Microscopy

Please note that all translations are automatically generated. Click here for the English version.

5.7K Views

•

08:47 min

•

January 25th, 2020

DOI :

10.3791/60784-v

January 25th, 2020

•

Avani V. Pisapati¹, Yi Wang*², Megan E. Blauch*³, Nathan J. Wittenberg³, Xuanhong Cheng¹^,², X. Frank Zhang¹^,⁴

¹Department of Bioengineering, Lehigh University, ²Department of Materials Science and Engineering, Lehigh University, ³Department of Chemistry, Lehigh University, ⁴Department of Mechanical Engineering and Mechanics, Lehigh University

Podsumowanie

We present a protocol for immobilizing single macromolecules in microfluidic devices and quantifying changes in their conformations under shear flow. This protocol is useful for characterizing the biomechanical and functional properties of biomolecules such as proteins and DNA in a flow environment.

Przeglądaj więcej filmów

Single molecule Conformational Changes

Fluorescence Microscopy

High Temporal Resolution

Microfluidic Device

Real time Visualization

Biomolecule Behavior

Rozdziały w tym wideo

0:05

Introduction

1:18

Preparation of PDMS Microfluidic Device

3:28

Microfluidic Device Surface Treatment

5:05

Fluorescence Microscopy

7:18

Results: Extension and Relaxation of VWF and Lambda DNA Molecules due to Shear Flow

8:00

Conclusion

Powiązane Filmy

article

Submillisecond Conformational Changes in Proteins Resolved by Photothermal Beam Deflection

8.9K Views

article

Flexural Rigidity Measurements of Biopolymers Using Gliding Assays

10.7K Views

article

Utilizing Time-Resolved Protein-Induced Fluorescence Enhancement to Identify Stable Local Conformations One α-Synuclein Monomer at a Time

3.1K Views

article

Using Microfluidics and Fluorescence Microscopy to Study the Assembly Dynamics of Single Actin Filaments and Bundles

2.5K Views

article

Monitoring Conformational Dynamics of Single Unmodified Proteins using Plasmonic Nanotweezers

272 Views

article

Examining the Conformational Dynamics of Membrane Proteins in situ with Site-directed Fluorescence Labeling

14.6K Views

article

Single-Molecule FRET Imaging for Observing the Conformational Dynamics of Dynamin-Like GTPase Atlastin

339 Views

article

Time-Resolved Fluorescence Anisotropy from Single Molecules for Characterizing Local Flexibility in Biomolecules

139 Views

article

Ensemble Force Spectroscopy by Shear Forces

1.4K Views

article

Single-Molecule Measurement of Protein Interaction Dynamics Within Biomolecular Condensates

3.2K Views

Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone

Używamy plików cookie, aby ulepszyć Twoje wrażenia z korzystania z naszej witryny.

Kontynuując korzystanie z naszej witryny lub klikając „Kontynuuj”, wyrażasz zgodę na akceptację naszych plików cookie.

Dowiedz się więcej