Labeling and Imaging of Amyloid Plaques in Brain Tissue Using the Natural Polyphenol Curcumin

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12.3K Views

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10:15 min

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November 1st, 2019

DOI :

10.3791/60377-v

November 1st, 2019

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Panchanan Maiti¹^,²^,³^,⁴^,⁵, Alexandra Plemmons¹, Zackary Bowers²^,³, Charles Weaver⁵, Gary Dunbar¹^,²^,³^,⁴

¹Field Neurosciences Institute, Ascension St. Mary's Hospital, ²Field Neurosciences Institute Laboratory for Restorative Neurology, Central Michigan University, ³Program in Neuroscience, Central Michigan University, ⁴Department of Psychology, Central Michigan University, ⁵Department of Health Sciences, Saginaw Valley State University

Podsumowanie

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Neurofibrillary Tangles

Alzheimer s Disease

Perfusion Procedure

21 Gauge Needle

Paraformaldehyde Solution

Cryostat Sectioning

Sucrose Solutions

Tissue Storage

Rozdziały w tym wideo

0:05

Title

1:04

Perfusion

2:24

Tissue Processing

3:48

Histochemical A-Beta Labeling of Cryostat Tissue Sections

5:00

Histochemical Labeling of Paraffin-Embedded Tissue Sections

5:42

A-Beta-Specific Antibody Labeling

7:24

Intracellular Amyloid-Beta Protein Colocalization

8:00

Results: Representative Curcumin-Amyloid Plaque Labeling and Imaging

9:24

Conclusion

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