JoVE Journal

Bioengineering

This content is Open Access.

Human iPSC-Derived Cardiomyocyte Networks on Multiwell Micro-electrode Arrays for Recurrent Action Potential Recordings

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11.4K Views

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08:53 min

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July 15th, 2019

DOI :

10.3791/59906-v

July 15th, 2019

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Viviana Zlochiver*¹, Stacie L. Kroboth*¹, Christopher R. Beal¹, Jonathan A. Cook¹, Rosy Joshi-Mukherjee¹^,²^,³

¹Aurora Research Institute, Advocate Aurora Health Care, ²Department of Biomedical Engineering, College of Engineering and Applied Science, University of Wisconsin-Milwaukee, ³Department of Medicine-Cardiovascular, School of Medicine, Johns Hopkins University

Podsumowanie

This article contains a set of protocols for the development of human induced pluripotent stem cell-derived cardiomyocyte (hiPSC-CM) networks cultured on multiwell MEA plates to reversibly electroporate the cell membrane for action potential measurements. High-throughput recordings are obtained from the same cell sites repeatedly over days.

Przeglądaj więcej filmów

IPSC Cardiomyocytes

Cardiomyocyte Networks

Multiwell MEA Plates

Action Potential Recordings

Electroporation

Electrophysiology Compounds

MATLAB Workflow

Cell Culture Incubator

Signal to noise Ratio

Quality Assurance

Fibronectin Dilution

Hydrophilic Treatment

Laminar Flow Hood

Rozdziały w tym wideo

0:04

Title

0:56

Pre-plating of the Cryopreserved hiPSC-CM for Maturation

1:35

Multiwell MEA Plate Sterilization and Coating

2:57

hiPSC-CM Dissociation and Plating on Multiwell MEA Plate

4:09

hiPSC-CM Electroporation and Signal Acquisition

5:06

Data Segmentation and Analysis

6:40

Results: Action Potential Recordings of hiPSC-CMs

8:21

Conclusion

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