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Neuroscience

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Nanyang Technological University

Conversion of Human Induced Pluripotent Stem Cells (iPSCs) into Functional Spinal and Cranial Motor Neurons Using PiggyBac Vectors

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11.2K Views

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07:33 min

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May 1st, 2019

DOI :

10.3791/59321-v

May 1st, 2019

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Maria G. Garone¹, Valeria de Turris², Alessandro Soloperto², Carlo Brighi², Riccardo De Santis³, Francesca Pagani²^,⁴, Silvia Di Angelantonio²^,⁴, Alessandro Rosa¹^,²

¹Department of Biology and Biotechnology Charles Darwin, Sapienza University of Rome, Italy, ²Center for Life Nano Science, Istituto Italiano di Tecnologia, Italy, ³Laboratory of Stem Cell biology and Molecular Embryology, The Rockefeller University, USA, ⁴Department of Physiology and Pharmacology, Sapienza University of Rome, Italy

Podsumowanie

This protocol allows rapid and efficient conversion of induced pluripotent stem cells into motor neurons with a spinal or cranial identity, by ectopic expression of transcription factors from inducible piggyBac vectors.

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PiggyBac Vectors

Transposable Vector

Amyotrophic Lateral Sclerosis

Skeletal Muscle Cells

Electroporation

Transgene Expression

Rozdziały w tym wideo

0:04

Title

0:54

Ngn2, Isl1 and Lhx3 (NIL) and Ngn2, Isl1 and Phox2a (NIP) Induced Pluripotent Stem Cell (iPSC) Line Generation

2:19

Antibiotic Selection

3:18

Motor Neuron Differentiation

5:12

Results: Representative Human iPSC Motor Neuron (MN) Differentiation Analysis

7:01

Conclusion

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