In Vitro Scratch Assay to Demonstrate Effects of Arsenic on Skin Cell Migration

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22.8K Views

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09:24 min

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February 23rd, 2019

DOI :

10.3791/58838-v

February 23rd, 2019

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Bronson I. Pinto¹^,⁴, Nathan D. Cruz¹, Oscar R. Lujan¹, Catherine R. Propper¹^,³, Robert S. Kellar¹^,²^,⁴

¹Department of Biological Sciences, Northern Arizona University, ²Department of Mechanical Engineering, Northern Arizona University, ³Department of Chemistry and Biochemistry, Northern Arizona University, ⁴Center for Bioengineering Innovation, Northern Arizona University

Podsumowanie

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In Vitro Scratch Assay

High throughput Screening

Biosafety Cabinet

Cryopreserved Cell Line

T75 Tissue Culture Flask

Media Supplementation

Incubation Conditions

Cell Adherence

Balanced Salt Solution

Rozdziały w tym wideo

0:04

Title

1:05

Human Dermal Fibroblasts Seeding in a T75 Flask

2:37

Cell Counting and Subculture into 12-Well Tissue Culture Plate

6:11

Scratch Assay Technique and Arsenic Contamination

7:44

Results: Analysis of the Scratch Assay

8:39

Conclusion

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