Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing

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12.2K Views

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08:51 min

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March 15th, 2019

DOI :

10.3791/58804-v

March 15th, 2019

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Lin Sun¹, Naoko Kono², Hiroyuki Toh³, Hanbing Xue¹, Kaori Sano⁴^,⁵, Tadaki Suzuki⁴, Akira Ainai⁴, Yasuko Orba⁶, Junya Yamagishi⁷^,⁸, Hideki Hasegawa⁴^,⁵, Yoshimasa Takahashi⁹, Shigeyuki Itamura², Kazuo Ohnishi⁹^,¹⁰

¹Graduate School of Life and Environmental Sciences, University of Tsukuba, ²Center for Influenza Virus Research, National Institute of Infectious Diseases, ³School of Science and Technology, Kwansei Gakuin University, ⁴Department of Pathology, National Institute of Infectious Diseases, ⁵Division of Infectious Diseases Pathology, Department of Global Infectious Diseases, Tohoku University Graduate School of Medicine, ⁶Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, ⁷Division of Collaboration and Education, Research Center for Zoonosis Control, Hokkaido University, ⁸Global Station for Zoonosis Control, GI-CoRE, Hokkaido University, ⁹Department of Immunology, National Institute of Infectious Diseases, ¹⁰Faculty of Life and Environmental Sciences, University of Tsukuba

Podsumowanie

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Antibody Repertoire

Next generation Sequencing

Immunoglobulin Amplicons

Rozdziały w tym wideo

0:04

Title

1:23

Nucleic Acid Isolation from Immune Cells and Tissues

3:04

cDNA Synthesis and PCR Amplification

4:58

NGS Sequencing of Libraries

5:33

Results: Identification of Mouse and Human Antibody Repertoires

8:11

Conclusion

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