Quantitative Immunoblotting of Cell Lines as a Standard to Validate Immunofluorescence for Quantifying Biomarker Proteins in Routine Tissue Samples

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8.7K Views

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09:58 min

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January 7th, 2019

DOI :

10.3791/58735-v

January 7th, 2019

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Alison M. Moore¹^,², Lee R. Boudreau³, Shakeel Virk³, David P. LeBrun¹^,²

¹Department of Pathology and Molecular Medicine, Queen's University, ²Division of Cancer Biology and Genetics, Queen's Cancer Research Institute, ³Queen's Laboratory for Molecular Pathology, Department of Pathology and Molecular Medicine, Queen's University

Podsumowanie

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Quantitative Immunoblotting

Protein Quantification

Biopsy Samples

Pathological Diagnosis

Cancer Treatments

Multiplexed Approach

Immortalized Cell Lines

Queen s Laboratory For Molecular Pathology

Centrifuge Procedure

Agarose Solution

Tissue Cassette

Rozdziały w tym wideo

0:04

Title

1:29

Building a Cell-Line Tissue Microarray (TMA)

3:37

Sample Staining by Immunofluorescence

4:27

Quantitative Immunoblotting of Cell Lines

7:48

Results: Immunoblotting Ratios Strongly and Positively Correlated with Intensity Readings from Quantitative IF

9:22

Conclusion

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