Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

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7.1K Views

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06:01 min

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January 7th, 2019

DOI :

10.3791/58451-v

January 7th, 2019

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Bushra Husain¹, Sairupa Paduchuri¹, Sree R. Ramani², Nadia Martinez-Martin¹

¹Receptor Discovery group, Microchemistry, Proteomics and Lipidomics Department, Genentech, ²Portfolio Management and Operations, Genentech

Podsumowanie

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Keywords Extracellular Protein Microarray

High Throughput Detection

Low Affinity Receptor ligand Interactions

Protein protein Interactions

Fc tagged Extracellular Domains

Protein A Coated Microbeads

Cy5 IgG Labeling

Rozdziały w tym wideo

0:04

Title

0:28

Extracellular Protein Microarray Printing

1:55

Preparation of Multivalent Bait Complexes

3:09

Extracellular Protein Microarray Screening

4:36

Results: Evaluation of Extracellular Protein Microarray Slide Printing and Screen of an Orphan Query Protein

5:11

Conclusion

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