Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy

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11.0K Views

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11:13 min

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August 20th, 2018

DOI :

10.3791/58167-v

August 20th, 2018

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Tanja D. Becke¹^,²^,³, Stefan Ness², Stefanie Sudhop¹^,³, Hermann E. Gaub³, Markus Hilleringmann², Arndt F. Schilling*⁴, Hauke Clausen-Schaumann*¹^,³

¹Center for Applied Tissue Engineering and Regenerative Medicine, Munich University of Applied Sciences, ²FG Protein Biochemistry & Cellular Microbiology, Munich University of Applied Sciences, ³Center for Nano Science, Ludwig-Maximilians-Universität München, ⁴Klinik für Unfallchirurgie, Orthopädie und Plastische Chirurgie, University Medical Center Göttingen

Podsumowanie

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Keywords Covalent Immobilization

Protein

Single Molecule Force Spectroscopy

Atomic Force Microscope

Cantilever Probe

Biomolecule Immobilization

Silanization

EDC

NHS

Phosphate buffered Saline

Rozdziały w tym wideo

0:04

Title

0:39

Immobilization of Proteins via Functional Silane Coupling Agents

4:42

Atomic Force Microscopy Based Single Molecule Force Spectroscopy

8:38

Results: Single Force Spectroscopy

10:07

Conclusion

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