Caffeine Extraction, Enzymatic Activity and Gene Expression of Caffeine Synthase from Plant Cell Suspensions
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09:11 min
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October 2nd, 2018
DOI :
October 2nd, 2018
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Rozdziały w tym wideo
0:04
Title
0:28
Caffeine Extraction in Cell Suspension of C. arabica L.
0:55
Conditions for the Assessment of Caffeine by Thin Layer Chromatography (TLC) Denistometry
1:29
Ribonucleic Acid (RNA) Isolation
2:55
Incubation of RNA Transcript with Deoxyribonuclease (DNase)
3:59
Complementary Deoxyribonucleic Acid (cDNA) Synthesis
5:03
Real-Time Quantitative PCR (qPCR) to Amplify the Gene CCS1
5:43
Total Protein of Cell Suspensions of C. arabica L.
6:26
Enzymatic Assay for Caffeine Synthase
7:39
Results: Enzymatic Activity of Caffeine Synthase Gene Evaluated via qPCR
8:30
Conclusion
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