Calibration-free In Vitro Quantification of Protein Homo-oligomerization Using Commercial Instrumentation and Free, Open Source Brightness Analysis Software

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7.2K Views

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08:22 min

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July 17th, 2018

DOI :

10.3791/58157-v

July 17th, 2018

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Rory Nolan¹, Luis A. Alvarez¹, Samuel C. Griffiths², Jonathan Elegheert², Christian Siebold², Sergi Padilla-Parra¹^,²^,³^,⁴

¹Cellular Imaging Group, Wellcome Centre Human Genetics, University of Oxford, ²Division of Structural Biology, Wellcome Centre Human Genetics, University of Oxford, ³Dynamic Structural Virology Group, Biocruces Health Research Centre, ⁴IKERBASQUE, Basque Foundation for Science

Podsumowanie

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Keywords Protein Homo oligomerization

In Vitro Quantification

Calibration free

Confocal Microscopy

Brightness Analysis

Protein protein Interactions

Drug Screening

Cancer Drugs

Small molecule Inhibitors

Fusion Inhibitors

Protein Dynamics

Cell cell Interactions

Size exclusion Chromatography

Rozdziały w tym wideo

0:04

Title

0:50

FKBP12F36V-mVenus Purification

3:24

Preparation of Multiwell Plate Array

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Calibration-free Confocal Acquisition

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Results: FKBP12 Dimerizes upon Addition of BB Dimerizer Drug

7:23

Conclusion

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