Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer

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12:23 min

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August 2nd, 2018

DOI :

10.3791/57996-v

August 2nd, 2018

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Larry C. Cheng*¹^,², Zhen Li*³, Thomas G. Graeber⁴, Nicholas A. Graham⁵, Justin M. Drake¹^,²^,³^,⁶^,⁷

¹Graduate Program in Cellular and Molecular Pharmacology, School of Graduate Studies, Rutgers University, The State University of New Jersey, ²Graduate Program in Quantitative Biomedicine, School of Graduate Studies, Rutgers University, The State University of New Jersey, ³Department of Medicine, Division of Medical Oncology, Rutgers Robert Wood Johnson Medical School, ⁴Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, Jonsson Comprehensive Cancer Center, UCLA Metabolomics Center, and California NanoSystems Institute, David Geffen School of Medicine, University of California, Los Angeles, ⁵Mork Family Department of Chemical Engineering and Materials Science, University of Southern California, ⁶Pharmacology, Rutgers Robert Wood Johnson Medical School, ⁷Cancer Metabolism and Growth Program, Rutgers Cancer Institute of New Jersey

Podsumowanie

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Keywords Phosphoproteome

Phosphopeptide Enrichment

Label free Quantitative Mass Spectrometry

Prostate Cancer

Signaling Networks

Therapeutic Resistance

Rozdziały w tym wideo

0:04

Title

0:38

Harvesting Tissue

1:31

Lysate Digestion and Reverse Phase Extraction

4:12

Immunoprecipitation and Enrichment of pY Peptides and Prewashing of pY Beads

6:59

Titanium Dioxide Enrichment of pY Peptides and Desalting for MS Analyses

9:32

Results: Prostate Cancer Phosphopeptide Enrichment and Analysis by Mass Spectrometry

11:29

Conclusion

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