Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity

0:41

Cell Culturing

1:37

4tU Labeling with S. pombe as a Spike-in

3:20

RNA Extraction and DNase Treatment

4:30

Thiol-specific Biotinylation of Newly Synthesized RNA

6:05

Purification of Newly Synthesized Fraction from Total and Unlabeled RNA Using Streptavidin-coated Magnetic Beads

7:18

Results: Quantification of Newly Synthesized mRNA as a Proxy for RNA Polymerase II Activity

8:52

Conclusion