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Developmental Biology

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Nanyang Technological University

A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue

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10.1K Views

•

06:17 min

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August 25th, 2018

DOI :

10.3791/57784-v

August 25th, 2018

•

Zhenan Liu¹, Jie Wen¹^,², Xue Leng¹, Qian Zhou¹, Changkuo Zhou³, Huaqiang Zhao¹, Xunwei Wu¹^,²

¹Shandong Provincial Key Laboratory of Oral Tissue Regeneration and Laboratory for Tissue Engineering and Regeneration, School of Stomatology, Shandong University, ²Suzhou Institute of Shandong University, ³Department of Urology, Qilu Hospital of Shandong University

Podsumowanie

Here we present a protocol to efficiently isolate primary human keratinocytes from adult skin tissues. This method simplifies the conventional procedure by using the ROCK Inhibitor Y-27632 in the inoculation medium to spontaneously separate epidermal cells from dermal cells.

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Primary Human Keratinocytes

Adult Skin Tissue

Simplified Method

Epidermal Cells

Laboratory Applications

Clinical Applications

Enzymatic Digestion

Keratinocyte Medium

Rozdziały w tym wideo

0:00

Title

0:20

Skin Tissue Pretreatment

1:07

Digestion of skin tissue

2:11

Collecting and culturing primary cells

3:42

Cell Passage

4:46

Representative results

6:06

Conclusion

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