Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions

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32.2K Views

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10:16 min

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June 28th, 2018

DOI :

10.3791/57320-v

June 28th, 2018

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Stefan Schoenfelder*¹, Biola-Maria Javierre*¹^,², Mayra Furlan-Magaril¹^,³, Steven W. Wingett¹^,⁴, Peter Fraser¹^,⁵

¹Nuclear Dynamics Programme, The Babraham Institute, Babraham Research Campus, ²IJC Building, Campus ICO-Germans Trias i Pujol, Josep Carreras Leukemia Research Institute, ³Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, ⁴Bioinformatics Group, The Babraham Institute, Babraham Research Campus, ⁵Department of Biological Science, Florida State University

Podsumowanie

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Keywords Promoter Capture Hi C

Genome wide Profiling

Promoter Interactions

Gene Regulatory Elements

Target Genes

Genome Structure

Gene Expression Control

Noncoding Disease Variants

Regulatory Control Sequences

Cell Lysis

Restriction Buffer

Cell Nuclei

Rozdziały w tym wideo

0:04

Title

0:52

Formaldehyde Fixation

2:14

HindIII Digestion and Biotinylation of Restriction Fragment Overhangs

4:09

Hybrid In-solution Capture

6:53

Isolation of Promoter Fragment-containing Ligation Products

8:55

Results: IL8 PCHi-C Profile in Human Hematopoietic Cells

9:34

Conclusion

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