Experimental Protocol for Detecting Mitochondrial Function in Hepatocytes Exposed to Organochlorine Pesticides

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7.8K Views

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08:39 min

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September 16th, 2020

DOI :

10.3791/56800-v

September 16th, 2020

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Qian Liu¹^,²^,³, Zhaoyan Jiang³, Aihua Gu¹^,²

¹State Key Laboratory of Reproductive Medicine, Institute of Toxicology, Nanjing Medical University, ²Key Laboratory of Modern Toxicology of Ministry of Education, School of Public Health, Nanjing Medical University, ³Center of Gallbladder Disease, Shanghai East Hospital, Institute of Gallstone Disease, Tongji University School of Medicine

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Keywords Mitochondrial Function

Hepatocytes

Organochlorine Pesticides

ATP Level

Oxygen Consumption

HepG2 Cells

Beta hexachlorcyclohexane

Mitochondrial Green Fluorescence Probe

Confocal Microscopy

Cellular ATP

Luminometer

Mitochondrial Membrane Potential

JC1 Dye

Rozdziały w tym wideo

0:04

Title

0:36

Mitochondrial Fluorescence Intensity and Cellular ATP Detection

2:45

Mitochondrial Membrane Potential Measurements

4:39

Oxygen Consumption Rate (OCR) Measurements

6:22

Results: Evaluation of Mitochondrial Function in Hepatocytes Exposed to β-HCH

7:43

Conclusion

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