Optimal Preparation of Formalin Fixed Samples for Peptide Based Matrix Assisted Laser Desorption/Ionization Mass Spectrometry Imaging Workflows

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7.9K Views

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08:16 min

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January 16th, 2018

DOI :

10.3791/56778-v

January 16th, 2018

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Matthew B. O'Rourke¹^,², Matthew P. Padula², Caine Smith³, Priscilla Youssef⁴, Stuart Cordwell¹, Paul Witting⁴, Greg Sutherland³, Ben Crossett¹

¹Mass Spectrometry Core Facility, University of Sydney, ²Proteomics Core Facility, University of Technology Sydney, ³Neuropathology Group, Discipline of Pathology, School of Medical Sciences, University of Sydney, ⁴Redox Biology Group, Discipline of Pathology, School of Medical Sciences, University of Sydney

Podsumowanie

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Keywords Formalin Fixed

Peptide Mass Spectrometry Imaging

Nitrocellulose Coating

Rozdziały w tym wideo

0:05

Title

0:29

Preparation of Reagents and Equipment

1:21

Preparation of Tissue Sections and Methylene Crosslink Hydrolysis

2:40

Tissue Digestion

3:57

Matrix Coating via Sublimation

5:26

Recrystallization and Instrumentation

6:20

Results: Correctly and Incorrectly Prepared Human Brain Tissue

7:07

Conclusion

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