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Behavior

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Nanyang Technological University

An Automated Rapid Iterative Negative Geotaxis Assay for Analyzing Adult Climbing Behavior in a Drosophila Model of Neurodegeneration

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9.8K Views

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06:52 min

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September 12th, 2017

DOI :

10.3791/56507-v

September 12th, 2017

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Wenze Cao*¹^,²^,³, Li Song*¹^,²^,³, Jingjing Cheng¹^,²^,³, Na Yi¹^,²^,³, Luyi Cai¹^,²^,³, Fu-de Huang⁴^,⁵, Margaret Ho¹^,²^,³

¹Research Center for Translational Medicine, Tongji University School of Medicine, ²Key Laboratory of Arrhythmias of the Ministry of Education of China, Shanghai East Hospital, Tongji University School of Medicine, ³Department of Anatomy and Neurobiology, Tongji University School of Medicine, ⁴Shanghai Advanced Research Institute, University of Chinese Academy of Sciences, Chinese Academy of Sciences, ⁵Sino-Danish College, University of Chinese Academy of Sciences, Chinese Academy of Sciences

Podsumowanie

This step-by-step protocol analyzes Drosophila negative geotaxis behavior using an automated multi-cylinder system that hosts hundreds of flies and synchronizes their action by an electric motor. Upon synchronization, fly negative geotaxis behavior is assayed, digitally recorded, and analyzed using the self-designed RflyDetection software.

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Neurodegeneration

Negative Geotaxis

Automated Behavior Analysis

Locomotive Activity

Rozdziały w tym wideo

0:05

Title

1:14

Fly Collection

2:13

Automated RING Assay

3:56

RING Assay Data Analysis

5:19

Results: Adult Climbing Behavior in Drosophila melanogaster

6:03

Conclusion

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