Long-term Live Imaging Device for Improved Experimental Manipulation of Zebrafish Larvae

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8.7K Views

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14:05 min

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October 27th, 2017

DOI :

10.3791/56340-v

October 27th, 2017

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Kayla Huemer*¹^,², Jayne M. Squirrell*³, Robert Swader², Kirsten Pelkey², Danny C. LeBert⁴, Anna Huttenlocher⁵^,⁶, Kevin W. Eliceiri¹^,²^,³

¹Department of Biomedical Engineering, University of Wisconsin-Madison, ²Morgridge Institute for Research, University of Wisconsin-Madison, ³Laboratory for Optical and Computational Instrumentation, University of Wisconsin-Madison, ⁴Cellular and Molecular Pathology Graduate Program, University of Wisconsin-Madison, ⁵Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, ⁶Department of Pediatrics, University of Wisconsin-Madison

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Keywords Zebrafish Larvae

Collagen Fiber Reorganization

Rozdziały w tym wideo

0:05

Title

0:57

Design and 3D Printing of Molds

2:57

PDMS Fabrication of zWEDGI Device

4:43

Plasma-bonding zWEDGI to Glass Dish

7:19

Channel Preparation and Loading Larvae

9:52

Wounding and Imaging Larvae and End of Experiment

11:14

Results: Post Wound Live Imaging and 3D Reconstruction of Zebrafish Larvae Tail Fin

12:36

Conclusion

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