JoVE Journal

Immunology and Infection

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Nanyang Technological University

Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions

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13.3K Views

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09:41 min

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October 17th, 2017

DOI :

10.3791/56293-v

October 17th, 2017

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Thomas A. Angelovich¹^,², Anna C. Hearps¹^,³, Anna Maisa¹, Theodoros Kelesidis⁴, Anthony Jaworowski¹^,³

¹Centre for Biomedical Research, Burnet Institute, ²School of Health and Biomedical Sciences, RMIT University, ³Department of Infectious Diseases, Monash University, ⁴University of California, Los Angeles

Podsumowanie

We describe a protocol to measure transmigration by monocytes across human endothelial monolayers and their subsequent maturation into foam cells. This provides a versatile method to assess the atherogenic properties of monocytes isolated from people with different disease conditions and to evaluate factors in blood which may enhance this propensity.

Przeglądaj więcej filmów

Monocyte Transmigration

Foam Cell Formation

Chronic Inflammatory Conditions

Cardiovascular Disease

Atherosclerosis

Endothelial Cells

Rozdziały w tym wideo

0:05

Title

0:50

Culturing HUVEC Monolayer on Collagen Gels

3:38

Activation of HUVEC Monolayer and Isolation/Activation of Monocytes for Transmigration

5:44

Quantification of Foam Cells and Macrophages by Microscopy or Flow Cytometry

7:42

Results: Characterizing the Transmigrating Mice

9:09

Conclusion

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