Sample Preparation for Endopeptidomic Analysis in Human Cerebrospinal Fluid

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10:23 min

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December 4th, 2017

DOI :

10.3791/56244-v

December 4th, 2017

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Karl T. Hansson¹, Tobias Skillbäck¹^,², Elin Pernevik¹, Jessica Holmén-Larsson¹, Gunnar Brinkmalm¹, Kaj Blennow¹^,², Henrik Zetterberg¹^,²^,³, Johan Gobom¹^,²

¹Inst. of Neuroscience and Physiology, Dept. of Psychiatry and Neurochemistry, Sahlgrenska Academy at University of Gothenburg, ²Clinical Neurochemistry Laboratory, Sahlgrenska University Hospital, ³Department of Molecular Neuroscience, UCL Institute of Neurology

Podsumowanie

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Keywords Endopeptidomic Analysis

Cerebrospinal Fluid

Sample Preparation

LC MS

Peptide Pre fractionation

Endogenous Peptides

Biomarkers

Neurodegenerative Disorders

MWCO Filtration

High pH Reverse Phase HPLC

Mass Spectrometry

Rozdziały w tym wideo

0:05

Title

1:12

Pre-treatment of Bulk Volume CSF without Quantification

2:24

Pre-treatment of Small Volume CSF Samples with Isobaric Labeling

4:00

MWCO Filtration, Desalting, and Sample Clean-up by Solid Phase Extraction

6:00

Offline High-pH Reverse Phase HPLC Sample Fractionation

7:09

LC-MS Analysis

7:47

Results: Peptide Identification After LC-MS Analysis

9:13

Conclusion

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