Flow Cytometry-based Drug Screening System for the Identification of Small Molecules That Promote Cellular Differentiation of Glioblastoma Stem Cells

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8.2K Views

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10:28 min

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January 10th, 2018

DOI :

10.3791/56176-v

January 10th, 2018

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Raffaella Spina¹, Dillon M. Voss¹, Laura Asnaghi², Andrew Sloan¹^,³, Eli E. Bar¹

¹Department of Neurological Surgery, Case Comprehensive Cancer Center, Case Western Reserve University School of Medicine, ²Department of Pathology, Johns Hopkins University, School of Medicine, ³Department of Neurological Surgery, University Hospital-Case Medical Center, Case Comprehensive Cancer Center, Case Western Reserve University School of Medicine

Podsumowanie

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Keywords Flow Cytometry

Drug Screening

Glioblastoma Stem Cells

Cellular Differentiation

Lentivirus

Reporter Construct

High throughput Screening

Small Molecules

Astroglial Differentiation

Cancer Stem Cells

Ehlers Danlos Glioblastoma

Cell Lineage

Bloodborne Pathogens

Neural Stem Cell Growth Medium

Polybrene

Dissociation Reagent

Rozdziały w tym wideo

0:05

Title

1:17

Generation of Lentiviral Transcriptional Reporter Cell Lines

3:47

Subclone Selection, Expansion, and Validation

4:47

Determination of GFAP:GFP Expression by Flow Cytometry

5:58

ELDA Self-renewal Assay to Assess Clonogenic Capacity

7:01

Drug Screen

8:27

Results: Selection and Drug Screening of Glioblastoma Stem Cell Subclones

9:46

Conclusion

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