JoVE Journal

Immunology and Infection

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Nanyang Technological University

Utilization of Capsules for Negative Staining of Viral Samples within Biocontainment

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10.1K Views

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08:22 min

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July 19th, 2017

DOI :

10.3791/56122-v

July 19th, 2017

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Candace D. Blancett¹, Mitchell K. Monninger¹, Chrystal A. Nguessan¹, Kathleen A. Kuehl¹, Cynthia A. Rossi², Scott P. Olschner², Priscilla L. Williams², Steven L. Goodman³, Mei G. Sun¹

¹Pathology Division, United States Army Medical Research Institute of Infectious Diseases (USAMRIID), ²Diagnostic Systems Division, United States Army Medical Research Institute of Infectious Diseases (USAMRIID), ³Microscopy Innovations LLC

Podsumowanie

This protocol provides instruction for negative staining virus samples which can easily be used in BSL-2, -3, or -4 laboratories. It includes the use of an innovative processing capsule, which protects the transmission electron microscopy grid and provides the user easier handling in the more turbulent environments within biocontainment.

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Electron Microscopy

Negative Staining

Potassium Phosphotungstic Acid

Osmium Tetroxide

Rozdziały w tym wideo

0:05

Title

1:08

Experiment Preparation in a BSL-2 Environment Prior to Working with the Virus Samples (Biocontainment Procedures were Filmed in BSL-4 Training Lab)

1:45

The Capsule Method for Negative Staining in Biocontainment Using Aqueous Glutaraldehyde and 1% Osmium Tetroxide Vapor Inactivation

4:24

The Capsule Method for Inactivation in Biocontainment 2% Glutaraldehyde Followed by Negative Staining in BSL-2 Laboratory

5:50

Results: Analyzing the Capsule Method of Negative Staining Viral Samples

7:14

Conclusion

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