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Biology

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Nanyang Technological University

Monitoring ER/SR Calcium Release with the Targeted Ca²⁺ Sensor CatchER⁺

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14.8K Views

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12:30 min

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May 19th, 2017

DOI :

10.3791/55822-v

May 19th, 2017

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Florence N. Reddish¹, Cassandra L. Miller¹, Rakshya Gorkhali¹, Jenny J. Yang¹

¹Department of Chemistry, Center of Diagnostics and Therapeutics (CDT), Georgia State University

Podsumowanie

We present protocols for the application of our targeted genetically-encoded calcium indicator (GECI) CatchER⁺ for monitoring rapid calcium transients in the endoplasmic/sarcoplasmic reticulum (ER/SR) of HEK293 and skeletal muscle C2C12 cells using real-time fluorescence microscopy. A protocol for the in situ K_d measurement and calibration is also discussed.

Przeglądaj więcej filmów

ER SR Calcium Release

Calcium Signaling

Calcium Transients

Cell Transfection

Fluorescence Imaging

Calcium related Diseases

Rozdziały w tym wideo

0:05

Title

11:32

Conclusion

10:14

Results: In Situ K_d of CatchER⁺ in C2C12 Myoblast Cells

1:30

Transfection of HEK293 Cells

2:43

Transfection of C2C12 Myoblast Cells

4:10

Preparation of Slide and Fluorescence Microscope

6:54

Imaging Drug-induced Ca²⁺ Transients and In Situ K_d Calibration

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