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Biology

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Nanyang Technological University

High-resolution Imaging and Analysis of Individual Astral Microtubule Dynamics in Budding Yeast

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9.2K Views

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10:23 min

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April 20th, 2017

DOI :

10.3791/55610-v

April 20th, 2017

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Colby P. Fees¹, Cassi Estrem¹, Jeffrey K. Moore¹

¹Department of Cell and Developmental Biology, University of Colorado School of Medicine

Podsumowanie

Budding yeast is an advantageous model for studying microtubule dynamics in vivo due to its powerful genetics and the simplicity of its microtubule cytoskeleton. The following protocol describes how to transform and culture yeast cells, acquire confocal microscopy images, and quantitatively analyze microtubule dynamics in living yeast cells.

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Microtubule Dynamics

GFP labeled Tubulin

Flow Chamber Slides

Time lapse Imaging

Rozdziały w tym wideo

0:05

Title

0:38

Growing Liquid Yeast Culture

9:32

Conclusion

8:15

Results: Astral Microtubule Dynamics in Altered in tub2-430Δ Mutant Cells

3:36

Loading Yeast Cells into Prepared Flow Chamber Slides

5:03

Image Acquisition

6:30

Image Analysis

1:26

Preparing Flow Chamber Slides

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