JoVE Journal

Immunology and Infection

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Nanyang Technological University

Protocols for Investigating the Host-tissue Distribution, Transmission-mode, and Effect on the Host Fitness of a Densovirus in the Cotton Bollworm

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8.4K Views

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11:12 min

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April 12th, 2017

DOI :

10.3791/55534-v

April 12th, 2017

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Xianming Yang¹, Pengjun Xu², Robert I. Graham³, He Yuan¹, Kongming Wu¹

¹State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, ²Tobacco Research Institute, Chinese Academy of Agricultural Sciences, ³Crop and Environment Sciences, Harper Adams University

Podsumowanie

Here, we present a protocol to investigate the host-tissue distribution, transmission mode, and effect on the host fitness of a densovirus within a lepidopteran species, the cotton bollworm. This protocol can also be used for studying the interaction between other orally-transmitted viruses and their insect hosts.

Przeglądaj więcej filmów

Cotton Bollworm

Virus host Interaction

Virus Transmission

Genetic Background

Single pair Mating

Virus Detection

Virus positive Strain

Virus negative Strain

Rozdziały w tym wideo

0:05

Title

0:40

Construction of the HaDV2-free Cotton Bollworm Colony

2:02

Preparation of HaDV2-containing Liquid Fluids

3:28

Construction of the HaDV2-infected Cotton Bollworm Colony

4:57

Tissue Distribution of HaDV2

7:01

Bioassays Testing the Effect of HaDV2 on Host Development

9:36

Results: Characterization of Effects of Densovirus on Bollworms

10:44

Conclusion

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