JoVE Journal

Biology

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Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells

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12.8K Views

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08:32 min

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March 16th, 2017

DOI :

10.3791/55486-v

March 16th, 2017

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Emrah Eroglu¹, Rene Rost¹, Helmut Bischof¹, Sandra Blass¹, Anna Schreilechner¹, Benjamin Gottschalk¹, Maria R. Depaoli¹, Christiane Klec¹, Suphachai Charoensin¹, Corina T. Madreiter-Sokolowski¹, Jeta Ramadani¹, Markus Waldeck-Weiermair¹, Wolfgang F. Graier¹, Roland Malli¹

¹Institute of Molecular Biology and Biochemistry, Medical University of Graz

Podsumowanie

This manuscript presents protocols for the application of novel genetically encoded nitric oxide (NO•) probes (geNOps) to monitor single cell NO• fluctuations in real-time using fluorescence microscopy. The Ca²⁺-triggered NO• formation on the level of individual endothelial cells was visualized by combining geNOps with a chemical Ca²⁺ sensor.

Przeglądaj więcej filmów

Genetically Encoded Fluorescent Nitric Oxide Probes

Real time Imaging

Nitric Oxide Signals

Fluorescence Microscope

Iron II Booster Solution

Perfusion Chamber

Gravity driven Perfusion

Physiological Calcium Buffer

Rozdziały w tym wideo

0:05

Title

1:07

Cell Preparation

2:33

Live Cell Imaging of NO• and Ca²⁺ Signals in Individual Cells

6:28

Results: Simultaneous Multichannel Imaging of NO• and Ca²⁺ Signals in Single EA.hy926 Cells

7:50

Conclusion

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