Determination of the Relative Potency of an Anti-TNF Monoclonal Antibody (mAb) by Neutralizing TNF Using an In Vitro Bioanalytical Method

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9.2K Views

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16:07 min

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September 16th, 2017

DOI :

10.3791/55376-v

September 16th, 2017

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Lilia Tierrablanca-Sánchez¹, Víctor Pérez Medina Martínez¹, Nancy D. Ramírez Ibañez¹, Néstor O. Pérez Ramírez¹, Francisco Luis Flores Ortiz¹, Emilio Medina-Rivero²

¹Research and Development, Probiomed, ²Bioprocess Research and Development Unit, Instituto Politecnico Nacional

Podsumowanie

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Keywords Anti TNF Monoclonal Antibody

TNF alpha Neutralization

In Vitro Bioanalytical Method

Spectrophotometric Analysis

Rozdziały w tym wideo

0:00

Title

0:31

Introduction

2:32

Methodology: Solutions

2:51

Methodology: Cells Thawing and First Subculturing

4:33

Methodology: Verification of Confluency

5:21

Methodology: Second Subculturing, Cells Detaching and Counting

7:45

Methodology: Antibody Quantitation and Dilutions

9:37

Methodology: Neutralization Assay

13:00

Methodology: Spectrophotometric Analysis

13:54

Results: Dose Response Curve

15:23

Conclusion

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