JoVE Journal

Developmental Biology

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Nanyang Technological University

Visualization of Cell Cycle Variations and Determination of Nucleation in Postnatal Cardiomyocytes

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8.7K Views

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09:41 min

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February 24th, 2017

DOI :

10.3791/55204-v

February 24th, 2017

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Alexandra Raulf*¹, Nadine Voeltz*¹, Daniel Korzus¹, Bernd K. Fleischmann¹, Michael Hesse¹

¹Institute of Physiology I, University of Bonn

Podsumowanie

To distinguish cell division from cell cycle variations in cardiomyocytes, we present protocols using two transgenic mouse lines: Myh6-H2B-mCh transgenic mice, for the unequivocal identification of cardiomyocyte nuclei, and CAG-eGFP-anillin mice, for distinguishing cell division from cell cycle variations.

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Multi nucleation

Enzyme Digestion

SiRNA Transfection

Rozdziały w tym wideo

0:05

Title

0:53

Cardiomyocyte Isolation

2:52

Cardiomyocyte siRNA Transfection and Confocal Video Microscopy Analysis

6:52

Results: Representative Cardiomyocyte Analyses

8:59

Conclusion

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