Methodology for the Study of Horizontal Gene Transfer in Staphylococcus aureus

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16.4K Views

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10:39 min

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March 10th, 2017

DOI :

10.3791/55087-v

March 10th, 2017

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Fabio Cafini*¹^,², Nguyen Thi Le Thuy*³, Federico Román⁴, José Prieto⁵, Sarah Dubrac⁶^,⁷, Tarek Msadek⁶^,⁷, Kazuya Morikawa¹

¹Division of Biomedical Science, Faculty of Medicine, University of Tsukuba, ²Department of Basic Biomedical Science, Universidad Europea de Madrid, ³Human Biology Program, School of Integrative and Global Majors, University of Tsukuba, ⁴Laboratory of Nosocomial Infections, Department of Bacteriology, Centro Nacional de MicrobiologÍa, Instituto de Salud Carlos III, ⁵Division of Microbiology, Department of Medicine, School of Medicine, Universidad Complutense, ⁶Biology of Gram-Positive Pathogens, Department of Microbiology, Institut Pasteur, Paris, France, ⁷ERL3526, CNRS, Paris, France

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Keywords Horizontal Gene Transfer

Staphylococcus Aureus

Conjugation

Transduction

Natural Transformation

Antibiotic Resistance

Rozdziały w tym wideo

0:05

Title

0:51

Conjugation Using the Filter-mating Method

3:10

Phage Amplification on the Donor

4:43

Measuring the Phage Titer

7:54

Transformation Assay

9:41

Results: Successful Horizontal Gene Transfer

10:10

Conclusion

6:01

Phage Transduction

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