A Protein Preparation Method for the High-throughput Identification of Proteins Interacting with a Nuclear Cofactor Using LC-MS/MS Analysis

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8.2K Views

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05:43 min

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January 24th, 2017

DOI :

10.3791/55077-v

January 24th, 2017

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Megumi Tsuchiya*¹, M. Rezaul Karim*², Taro Matsumoto³, Hidesato Ogawa¹, Hiroaki Taniguchi³^,⁴^,⁵

¹Graduate School of Frontier Biosciences, Osaka University, ²Department of Biotechnology and Genetic Engineering, Jahangirnagar University, ³Division of Cell Regeneration and Transplantation, School of Medicine, Nihon University, ⁴Institute of Genetics and Animal Breeding of the Polish Academy of Sciences, ⁵Graduate School of Life and Medical Sciences, Doshisha University

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Keywords Protein Preparation

Transcriptional Mechanisms

Rozdziały w tym wideo

0:05

Title

0:45

Protein Extraction

2:01

Immunoprecipitation

3:47

Alkylation and Trypsinization

4:46

Results: Purification of ARIP4 Complexes

5:13

Conclusion

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