Use of a Filter Cartridge for Filtration of Water Samples and Extraction of Environmental DNA

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24.8K Views

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08:04 min

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November 25th, 2016

DOI :

10.3791/54741-v

November 25th, 2016

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Masaki Miya¹, Toshifumi Minamoto², Hiroki Yamanaka³, Shin-ichiro Oka⁴, Keiichi Sato⁴, Satoshi Yamamoto², Tetsuya Sado¹, Hideyuki Doi⁵

¹Department of Ecology and Environmental Sciences, Natural History Museum and Institute, Chiba, ²Graduate School of Human Development and Environment, Kobe University, ³Faculty of Science and Technology, Ryukoku University, ⁴Okinawa Churashima Research Center, ⁵Graduate School of Simulation Studies, University of Hyogo

Podsumowanie

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Keywords Filter Cartridge

Water Filtration

Environmental DNA

Metabarcoding

Biodiversity Monitoring

Rozdziały w tym wideo

0:05

Title

0:46

Assembly of the Filtration System

1:37

Filtration of Water Samples ≤4 L Using the Filter Cartridge

3:05

Filtration of Water Samples >4 L Using the Filter Cartridge

4:23

Extraction of eDNA from the Filter

6:57

Results: MiFish Metabarcoding of eDNA

7:23

Conclusion

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