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Developmental Biology

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Nanyang Technological University

Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program

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7.1K Views

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11:00 min

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December 16th, 2016

DOI :

10.3791/54372-v

December 16th, 2016

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Michael G. Daniel¹^,²^,³, Carlos-Filipe Pereira⁴, Jeffrey M. Bernitz¹^,²^,³, Ihor R. Lemischka¹^,³^,⁵, Kateri Moore¹^,³

¹Department of Developmental and Regenerative Biology, Icahn School of Medicine at Mount Sinai, ²The Graduate School of Biomedical Science, Icahn School of Medicine at Mount Sinai, ³Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, ⁴Center for Neuroscience and Cell Biology, University of Coimbra, ⁵Department of Pharmacology and Systems Therapeutics, Icahn School of Medicine at Mount Sinai

Podsumowanie

The protocol described here details the induction of a hemogenic program in mouse embryonic fibroblasts via overexpression of a minimal set of transcription factors. This technology may be translated to the human system to provide platforms for future study of hematopoiesis, hematologic disease, and hematopoietic stem cell transplant.

Przeglądaj więcej filmów

Fibroblast Reprogramming

Transcription Factors

Hemogenic Induction

Hematopoietic Precursor Cells

Multi lineage Differentiation

Gelatin Coating

Virus Transduction

Doxycycline Induction

Hematopoietic Culture Medium

Cell Dissociation

Rozdziały w tym wideo

0:05

Title

0:54

Mouse Embryonic Fibroblast Reprogramming

4:02

Placental Aggregation and Colony Forming Unit Assays in Methylcellulose

8:32

Results: Transcription Factor-induced Hemogenic Programming of Mouse Embryonic Fibroblasts

9:58

Conclusion

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