Large-Scale Production of Cardiomyocytes from Human Pluripotent Stem Cells Using a Highly Reproducible Small Molecule-Based Differentiation Protocol

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10.5K Views

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12:21 min

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July 25th, 2016

DOI :

10.3791/54276-v

July 25th, 2016

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Hananeh Fonoudi*¹^,²^,³^,⁸, Hassan Ansari*¹^,⁸, Saeed Abbasalizadeh¹, Gillian M Blue⁶^,⁷, Nasser Aghdami¹, David S Winlaw⁶^,⁷, Richard P Harvey²^,³^,⁴, Alexis Bosman*²^,³, Hossein Baharvand*¹^,⁵

¹Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, ²Developmental and Stem Cell Biology Division, Victor Chang Cardiac Research Institute, ³St. Vincent´s Clinical School, Faculty of Medicine, University of New South Wales, ⁴School of Biotechnology and Biomolecular Sciences, University of New South Wales, ⁵Department of Developmental Biology, University of Science and Culture, ⁶Heart Centre for Children, The Children´s Hospital at Westmead, ⁷Sydney Medical School, University of Sydney, ⁸Department of Developmental Biology, University of Science and Culture, Tehran, Iran

Podsumowanie

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Keyword Extraction Cardiomyocytes

Human Pluripotent Stem Cells

Differentiation Protocol

High Efficiency

Reproducibility

Cardiac Disease Modeling

Human Induced Pluripotent Stem Cells

Human Embryonic Stem Cells

Single Cell Passaging

Rozdziały w tym wideo

1:03

Spinner Flask Internal Surface Silicon Coating

0:05

Title

2:42

hPSC Spheroid Stirred Suspension Bioreactor Differentiation

6:42

hPSC Differentiation Using Cultures Not Adapted to Single Cell Passaging

9:46

Results: Representative Cardiomyocyte Differentiation From Various hPSCs Cultures

11:19

Conclusion

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