Isolation of Perivascular Multipotent Precursor Cell Populations from Human Cardiac Tissue

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7.3K Views

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08:15 min

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October 8th, 2016

DOI :

10.3791/54252-v

October 8th, 2016

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James E. Baily¹, William C.W. Chen²^,³, Nusrat Khan⁴, Iain R. Murray⁴, Zaniah N. González Galofre⁴, Johnny Huard⁵^,⁶, Bruno Péault⁴^,⁷

¹Centre for Cardiovascular Science, Queen's Medical Research Institute, University of Edinburgh, ²Department of Bioengineering and Orthopaedic Surgery, University of Pittsburgh, ³Research Laboratory of Electronics and Department of Biological Engineering, Massachusetts Institute of Technology, ⁴MRC Centre for Regenerative Medicine, University of Edinburgh, ⁵Stem Cell Research Center, Department of Orthopaedic Surgery, University of Pittsburgh, ⁶Department of Orthopaedic Surgery, University of Texas Health Science Center at Houston, ⁷Department of Orthopaedic Surgery, UCLA Orthopaedic Hospital, David Geffen School of Medicine, University of California at Los Angeles

Podsumowanie

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Keywords Perivascular

Ischemic Heart Disease

Cardiomyogenic Potential

Rozdziały w tym wideo

0:05

Title

1:06

Tissue Digestion and Cell Isolation

3:27

Cell Labelling and Sorting

6:05

Cell Culture

6:54

Results: FAS Purification of Myocardial Perivascular Cells and Perivascular Cell Morphology

7:39

Conclusion

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