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siRNA Transfection and EMSA Analyses on Freshly Isolated Human Villous Cytotrophoblasts

10.5K Views

09:57 min

September 20th, 2016

DOI :

10.3791/53995-v

September 20th, 2016


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SiRNA Transfection

Rozdziały w tym wideo

0:05

Title

1:00

Evaluation of the Purity of Isolated Primary Villous Cytotrophoblasts

3:13

siRNA Transfection of Human Primary Villous Cytotrophoblasts

4:55

Preparation of Nuclear Extracts from Cultured Cells

5:52

Radiolabeling of Single Stranded Oligonucleotide

6:50

DNA Binding Reaction

8:07

Results: Syncytin-2 Knowckdown and Visualization of Syncytin-2 DNA-protein Complexes

9:14

Conclusion

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