An ELISA Based Binding and Competition Method to Rapidly Determine Ligand-receptor Interactions

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18.9K Views

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08:40 min

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March 14th, 2016

DOI :

10.3791/53575-v

March 14th, 2016

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Mohameedyaseen Syedbasha¹, Janina Linnik¹^,²^,³, Deanna Santer⁴, Daire O'Shea⁵, Khaled Barakat⁴^,⁶, Michael Joyce⁴, Nina Khanna⁷, D. Lorne Tyrrell⁴, Michael Houghton⁴, Adrian Egli¹^,⁸

¹Applied Microbiology Research, Department of Biomedicine, University of Basel, ²Department of Biosystems Science and Engineering, ETH Zurich, and Swiss Institute of Bioinformatics, ³Swiss Institute of Bioinformatics, ⁴Li Ka Shing Institute for Virology, University of Alberta, ⁵Regional Infectious Diseases Unit, University of Edinburgh, ⁶Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, ⁷Infection Biology, Department of Biomedicine, University of Basel, ⁸Clinical Microbiology, University Hospital Basel

Podsumowanie

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Keywords ELISA

Binding Affinity

Competition Assay

Ligand receptor Interaction

Protein protein Interactions

Cytokine receptor Binding

Blocking Compounds

Receptor Coating

Plate Blocking

Rozdziały w tym wideo

0:05

Title

1:07

Preparations and Introduction of Assays

2:14

Direct Ligand-Receptor-Interaction Assay (Direct LRA)

5:05

Competition Ligand-Receptor-Interaction Assay (Competition LRA)

6:24

Results: Binding Between INFL 1-3 Peptides and Their Receptor Sub-unit IL28RA

7:48

Conclusion

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