Establishment of a Human Multiple Myeloma Xenograft Model in the Chicken to Study Tumor Growth, Invasion and Angiogenesis

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13.0K Views

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10:04 min

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May 1st, 2015

DOI :

10.3791/52665-v

May 1st, 2015

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Agnieszka Martowicz*¹^,⁴, Johann Kern*¹^,², Eberhard Gunsilius¹, Gerold Untergasser¹^,³

¹Department of Internal Medicine V, Innsbruck Medical University, ²Oncotyrol GmbH, ³Tyrolean Cancer Research Institute, ⁴Division of Vascular Biology, Department of Medical Biochemistry and Biophysics, Karolinska Institute

Podsumowanie

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Keywords Multiple Myeloma

Xenograft Model

Chicken Chorioallantoic Membrane

Therapeutic Screening

Rozdziały w tym wideo

0:05

Title

2:09

Cell Culture and Lentiviral Transfection

3:20

3D-Multiple Myeloma Spheroid Model

4:47

3D Multiple Myeloma Xenograft Model in the CAM

6:17

Quantification of eGFP Protein by ELISA

7:03

Immunohistochemical Analysis of Blood Vessels and Invading Tumor Cells

8:05

Results: Bortezomib Treatment of Multiple Meyloma Xenografts

9:17

Conclusion

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