Intracerebroventricular Viral Injection of the Neonatal Mouse Brain for Persistent and Widespread Neuronal Transduction

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58.5K Views

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10:15 min

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September 15th, 2014

DOI :

10.3791/51863-v

September 15th, 2014

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Ji-Yoen Kim*¹, Stacy D. Grunke*¹, Yona Levites², Todd E. Golde², Joanna L. Jankowsky¹^,³

¹Department of Neuroscience, Baylor College of Medicine, ²Department of Neuroscience, Center for Translational Research in Neurodegenerative Disease, University of Florida, ³Department of Neurology and Department of Neurosurgery, Baylor College of Medicine

Podsumowanie

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Keywords Intracerebroventricular

Viral Injection

Neonatal Mouse Brain

Neuronal Transduction

Adeno associated Virus

Rozdziały w tym wideo

0:05

Title

1:24

Staging the Newborn Pups for Injection

2:38

Free-hand Intracranial Injection of AAV into Neonatal Mice

4:54

Stereotaxic Injection of AAV into Neonatal Mice

7:21

Post-injection Care

7:54

Effect of Viral Titer and Serotype on VIral Transduction and Co-expression

9:55

Conclusion

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