Preparation of Primary Neurons for Visualizing Neurites in a Frozen-hydrated State Using Cryo-Electron Tomography

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79.1K Views

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09:59 min

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February 12th, 2014

DOI :

10.3791/50783-v

February 12th, 2014

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Sarah H. Shahmoradian¹, Mauricio R. Galiano², Chengbiao Wu³, Shurui Chen⁴, Matthew N. Rasband², William C. Mobley³, Wah Chiu⁴

¹Department of Molecular Physiology and Biophysics, Baylor College of Medicine, ²Department of Neuroscience, Baylor College of Medicine, ³Department of Neuroscience, University of California at San Diego, ⁴National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine

Podsumowanie

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Keywords Neurites

Primary Neurons

Cryo electron Tomography

Neurological Disorders

Rozdziały w tym wideo

0:05

Title

1:19

Preparing Dishes with EM Grids

3:43

Preparing and Plating Primary Neurons on EM Grids

4:45

Vitrifying Neurons on EM Grids

8:05

Results: Visualizing Neurites in a Frozen-hydrated State Using Cryo-Electron Tomography

9:32

Conclusion

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